Cryo-tomography tilt-series alignment with consideration of the beam-induced sample motion
                    
                        
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                    چکیده
منابع مشابه
Beam-induced motion correction for sub-megadalton cryo-EM particles
In electron cryo-microscopy (cryo-EM), the electron beam that is used for imaging also causes the sample to move. This motion blurs the images and limits the resolution attainable by single-particle analysis. In a previous Research article (Bai et al., 2013) we showed that correcting for this motion by processing movies from fast direct-electron detectors allowed structure determination to near...
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Transmission electron microscopy allows the collection of multiple views of specimens and their computerized three-dimensional reconstruction and analysis with electron tomography. Here we describe development of methods for automated multi-tilt data acquisition, tilt-series processing, and alignment which allow assembly of electron tomographic data from a greater number of tilt series, yieldin...
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Fast-moving visual features are thought to leave neural 'streaks' that can be detected by orientation-selective cells. Here, we tested whether 'motion streaks' can induce classic tilt aftereffects (TAEs) and tilt illusions (TIs). For TAEs, participants adapted to random arrays of small Gaussian blobs drifting at 9.5 deg/s. Following adaptation to directions of 15, 30, 45, 60, 75, and 90 degrees...
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Transmission electron microscopy, combined with tomographic techniques, has been used for decades to generate 3D representations of biological structures, with resolutions down to the nanometer range [1,2]. Here, we describe a method, extending the Transform-Based Reconstruction (TxBR) method [3,4] whereby large field electron tomography (ET) can be optimized both in terms of data acquisition a...
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ژورنال
عنوان ژورنال: Journal of Structural Biology
سال: 2018
ISSN: 1047-8477
DOI: 10.1016/j.jsb.2018.02.001